Semnan University Press Applied Chemistry Today 2981-2437 19 73 2024 11 21 Efficient pre-concentration and determination of toxic metals utilizing the tandem air-agitated liquid-liquid microextraction method with a deep eutectic solvent as a brand-new and eco-friendly media in biological and water samples Efficient pre-concentration and determination of toxic metals utilizing the tandem air-agitated liquid-liquid microextraction method with a deep eutectic solvent as a brand-new and eco-friendly media in biological and water samples 161 176 9055 10.22075/chem.2024.31416.2196 FA Nooshin Ghassab Department of Analytical Chemistry, Faculty of Chemistry, Semnan University, Semnan, Iran Maryam Rajabi Department of Analytical Chemistry, Faculty of Chemistry, Semnan University, Semnan, Iran Alireza Asghari Department of Analytical Chemistry, Faculty of Chemistry, Semnan University, Semnan, Iran Journal Article 2023 07 31 In this work, we present a novel extracting media named as deep eutectic solvent containing choline chloride and cinnamyl alcohol (ChCl: Cinnamyl alcohol), that has unique properties such as simple and cheap synthesize, eco-friendly, and bio-degradability. 2-amino-5-mercapto-1,3,4-thiadiazole was used as the chelating agent. Then, it was efficiently employed at the technique of Tandem air-agitated liquid-liquid microextraction (TAALLME) of metal ions (Pb2+ and Cd2+) in biological and aqueous samples pursued by inductively coupled plasma-optical emission spectrometry (ICP-OES). This secure, impressive, and speedy pre-concentration procedure based on the modern DES was quickly practical with no extra requirement the emulsifier intermediates. Effective statistical optimization of basic variables results by the Central Composite Design (CCD) demonstrated that pH of acceptor phase 7.00, pH of donor phase 2.00, 1175µL of acceptor solvent, and 325µL of extraction solvent be consequented on topmost extraction recoveries. In optimum conditions, the reliable analytical features were found to be: large range of linearity (LDRs) 0.65-250 and 0.065-250 ng mL−1, low limits of detection (LODs) of 0.2 and 0.02 ng mL−1, and rational precision (%RSDs, n=5) of 2.98 and 2.93% for Pb2+ and Cd2+, respectively. Also, recoveries of extraction and enrichment factors were 97-98% and 41.84, respectively. These sufficiently good results confirmed the powerful efficacy of the suggested microextraction technique for achievement to pure and proper enrichment of the aforementioned compounds in highly complex real samples. In this work, we present a novel extracting media named as deep eutectic solvent containing choline chloride and cinnamyl alcohol (ChCl: Cinnamyl alcohol), that has unique properties such as simple and cheap synthesize, eco-friendly, and bio-degradability. 2-amino-5-mercapto-1,3,4-thiadiazole was used as the chelating agent. Then, it was efficiently employed at the technique of Tandem air-agitated liquid-liquid microextraction (TAALLME) of metal ions (Pb2+ and Cd2+) in biological and aqueous samples pursued by inductively coupled plasma-optical emission spectrometry (ICP-OES). This secure, impressive, and speedy pre-concentration procedure based on the modern DES was quickly practical with no extra requirement the emulsifier intermediates. Effective statistical optimization of basic variables results by the Central Composite Design (CCD) demonstrated that pH of acceptor phase 7.00, pH of donor phase 2.00, 1175µL of acceptor solvent, and 325µL of extraction solvent be consequented on topmost extraction recoveries. In optimum conditions, the reliable analytical features were found to be: large range of linearity (LDRs) 0.65-250 and 0.065-250 ng mL−1, low limits of detection (LODs) of 0.2 and 0.02 ng mL−1, and rational precision (%RSDs, n=5) of 2.98 and 2.93% for Pb2+ and Cd2+, respectively. Also, recoveries of extraction and enrichment factors were 97-98% and 41.84, respectively. These sufficiently good results confirmed the powerful efficacy of the suggested microextraction technique for achievement to pure and proper enrichment of the aforementioned compounds in highly complex real samples. https://chemistry.semnan.ac.ir/article_9055_158fd8349884cc05b82ccbad0353af89.pdf