Application of dispersive liquidâliquid microextraction and high-performance liquid chromatography for the determination of cetrimonium bromide in water samples

Rajabi, Maryam; Ghazaghi, Mehri

doi:10.22075/chem.2017.638

Application of dispersive liquidâliquid microextraction and high-performance liquid chromatography for the determination of cetrimonium bromide in water samples

Authors

Maryam Rajabi

Mehri Ghazaghi

10.22075/chem.2017.638

Abstract

A simple, rapid and sensitive
method for the determination of cetrimonium bromide at trace levels in water
samples was developed by combining a dispersive liquid âliquid
microextraction (DLLME) technique with high performance liquid chromatography
(HPLC-UV). A mixture of extraction solvent and dispersive solvent were
rapidly injected into 10.0 mL aqueous sample, and a cloudy solution was
formed. After extraction of the analyte into the fine droplets of extractant,
phase separation was performed by centrifugation and the enriched analyte in
the sedimented phase was determined by HPLC-UV. The factors relevant to the
microextraction efficiency, such as the kind and volume of extraction and
dispersive solvent, and the extraction time were optimized. Under the optimum
conditions (extraction solvent: chlorobenzene, volume, 70 Î¼L dispersive
solvent: ethanol, volume, 0.75 mL), the enrichment factor of 200 was
obtained. The linear range was 0.005â100 Âµg L-1. The relative
standard deviation of 5.9% (n= 5), and detection limit (signal-to-noise ratio
of 3) of 0.10 pg mL-1 were obtained.

Keywords

Keywords: Dispersive liquid-liquid microextraction

Cetrimonium bromide

High performance liquid chromatography