Green optical sensor based on saffron for monitoring and determination of nitrite in the environmental samples

Document Type : Original Article

Authors

Department of Chemistry, Zanjan University, Zanjan, Iran

Abstract

Optical chemical sensors (optode) have important place in the analytical chemistry research area because of their use in different fields of human knowledge’s. The study of these sensors is also attractive since they allow us to perform in vivo analysis and in situ monitoring. Determination of nitrite ions as a potentially carcinogenic species is in the core attention of the analytical scientists to produce simple, fast, cost effective and environmental friendly approaches. In this work a new simple, low cost, and sensitive optical sensor has been developed for determination of nitrite ions based on the immobilization of saffron (Crocus sativus L.) as a natural sensing reagent on the triacetylcellulose membrane. Selective reaction of the saffron and nitrite ions in the acidic media was monitored photometrically and absorption changes in the wavelength of 440 nm were recorded as analytical signal. The influence of effective variables on the optode performance including triacetylcellulose activation process, concentration of saffron, response time and the effect of ionic strength were studied and optimized. In the optimum conditions, the sensor has good durability and a short response time with linear dynamic range of 1.010-3 – 50.0 μg mL-1 and detection limits of 7.010-4 μg mL-1. Reproduce determinations were done on the solution containing 5.0 μg mL-1 nitrite ion and RSD (n=5) of 4 % was obtained. The effects of various ions and species on the analytical signal were also studied and application of the proposed method in the determination of nitrite ion in many real samples such as tap and well water gave satisfactory results.

Keywords


[1] Shahrasbi, Hamzeh; Naseri, Ali; Nutritional value and practical methods of health and chemical control of some Iranian meat products, Jihad Daneshgahi Publications, first edition (1985).
[2] Nowruz S, PhD thesis, Isfahan University of Technology, July 2005.
[3] Nowruz S, M.Sc. Thesis, Tarbiat Modares University, Summer 1995.
[4] M. R. Burkart, D. W. Kolpin, R. J.  Jaquis, and K. J. Cole, Environ. Qual., 28) 1999 (1908.
[5] Nouroozi, S., Aghamohammadi, M., “Reverse flow injection analysis of trace amounts of nitrite using saffron as natural reagent”, J. Appl. Chem., 1396, (2019), 17.
[6] Pirsaheb; M., Sharafi; K., Morad M., “A survey on nitrite and nitrate levels in vegetables and cucurbits cultivated in northern and western plains of Kermanshah city in 2012” J. Food Hygiene 3, 1(9) (2013) 77.
[7] W. Fresenius, K. E. Quentin, W. Schneider, “Water Analysis”, Wiley, New York, (1988), 226.
[8] Ensafi, Ali A., Rezaei, B. and Nouroozi, S., “Simultaneous spectrophotometric determination of nitrite and nitrate by flow injection analysis”, Anal. Sci., 20 (1) (2004) 1749.
[9] Nouroozi, S. and R. Mirshafian, “Flow injection kinetic spectrophotometric method for the determination of trace amounts of nitrite”, Talanta, 79 (2009) 1149.
[10] Amini, M., M. Pourhossein, and M. Talebi, “A chemiluminescence flow injection system for nitrite ion determination”, J. Irani. Chem. Soc, 2 (2005) 305.
[11]. M. F. Mousavi, A. Jabbari, S. Nouroozi, “A Sensitive Flow Injection Method for Determination of Trace amounts of Nitrite " Talanta, 45 (1998) 1247.
[12] Scog, eh. Douglas; Haller, James. F; Nimen, eh. Timothy; Principles of Instrumental Analysis, translated by Abdolreza Selajgeh, Tehran, University Publishing Center, Fourth Edition (2008).
[13] Ensafi Ali A., Kazemzadeh A., “Monitoring nitrite with optical sensing films” Microchem. J., 72 (2002) 193.
[14] Kazemzadeh A., Daghighi S., “Optical nitrite sensor based on chemical modification of a polymer film”, Spectrochim. Acta, A Mol. Biomol. Spectrosc., 61 (2005) 1871.
[15] Ensafi Ali A., Amini M., “A highly selective optical sensor for catalytic determination of ultra-trace amounts of nitrite in water and foods based on brilliant cresyl blue as a sensing reagent” Sensor Actuat B-Chem., 147 (2010) 61.
[16] Abdukayum A., “Optical Sensor Based on Phenosafranine Doped Organically Modified Sol-Gel Film for Nitrite”, Chin. J. Anal. Chem, 39 (2011) 564.
[17] Dhanya S., Joy J., Rao T.P., “Fabrication and characterization of Rhodamine 6G entrapped sol–gel film test strip for virtually specific and sensitive sensing of nitrite” Sensor Actuat B-Chem., 173 (2012) 510.
[18] Ensafi Ali A., Amini M., “Highly selective optical nitrite sensor for food analysis based on Lauth's violet-triacetyl cellulose membrane film” Food Chem., 132 (2012) 1600.
[19] Afkhami A., Madrakian T., Aleseyyed S.B., “Kinetic Determination of Trace Amounts of Nitrite Using an Optical Chemical Sensor”, Clean-Soil Air Water, 40 (2012) 619.
[20] Greenwood N. N.; Earnshaw A. (1997). Chemistry of the Elements (2nd ed.). Butterworth-Heinemann. 461.
[21] Skoog D. A., West D. M.,(1982). Fundamentals of Analytical Chemistry (4th ed.).CBS Publishing, HRW International editions. 65.