Determination of Triton X-100 in water samples using dispersive liquid-liquid microextraction and HPLC with the aid of response surface methodology



An improved method for the determination of Triton X-100 in water samples has been developed using dispersive liquid–liquid micro extraction (DLLME) prior to HPLC analysis. The volume of extraction solvent (CHCl3), disperser solvent (acetone) and sample solution, pH, ionic strength, and extraction time were selected as interested variables in DLLME process. Firstly, a screening experiment, Taghochi two-level orthogonal array, was employed to identify the important factors. Then the elucidation and optimization relationship between the response and the important factors was investigated by response surface methodology (RSM). The central composite design as the most popular of the many classes of RSM designs was used. The optimum experimental conditions found from this statistical evaluation were included: sample volume: 10.0 mL, volume of extraction solvent (chloroform): 137.7 µL, volume of disperser solvent (acetone): 0.6 mL, centrifugation time: 5.0 min, pH: 6.2 (natural pH) , 2.5% (w/v) NaCl and extraction time: 6.0 min. Under the optimum conditions, the preconcentration factor of 120 was achieved. Calibration graph was linear in the range of 0.01-100.0 mg L−1 with correlation coefficient of 0.9961. The Limit of detection (LOD) of 3.0 µg L-1 was obtained for Triton X-100 determination