Simultaneous Determination of Ascorbic Acid, Dopamine, and Uric Acid by Differential Pulse Voltammetry Using Poly(Chromazurol s) Modified Glassy Carbon Electrode



The Chromazurol s has been electropolymerized on the glassy carbon electrode and the Poly- Chromazurol s modified glassy carbon electrode (PCSMGCE) has shown excellent electrocatalytic activity toward the oxidation of ascorbic acid in phosphate buffer solution (pH=3). It was used for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA). Analytical parameters were optimized and electrochemical behavior of modified electrode was studied. The calibration curves were obtained over the range of 3.0-2200.0 µmol L–1 AA, 0.09-980.0 µmol L–1 DA, and 0.05-900.0 µmol L–1 UA. Detection limits of 1.7 µmol L–1 AA, 0.05 µmol L–1 DA, and 0.03 µmol L–1 UA were obtained at pH 3.0. Prepared modified electrode had satisfactory results for determination of DA, AA and UA in dopamine injection solution, vitamin C tablets and human urine samples.