Green synthesis of metal nanoparticles using aqueous extract of Moringa oleifera L. and investigating their antioxidant and antibacterial properties

Document Type : Original Article


1 Faculty of Agriculture and Natural Resources, Department of Plant Production and Genetics, Mohaghegh Ardabili University, Ardabil, Iran

2 Faculty of Pharmacy, Department of Pharmaceutics, University of Medical Sciences, Ardabil Branch, Ardabil, Iran

3 Faculty of Pharmacy, Department of Pharmacognosy, University of Medical Sciences, Ardabil Branch, Ardabil, Iran


Moringa plant with the scientific name Moringa oleifera L. has important medicinal chemical compounds including flavonoids, which have antioxidant and anticancer properties. In this research, the properties of metal nanoparticles such as Iron, Copper, Zinc and Silver biosynthesized from the aqueous extract of M. oleifera plant have been studied, and then the antioxidant and antibacterial properties of the aqueous extract obtained from the aerial parts of this plant and the nanoparticles have been studied. The resulting metal was treated. The size and structure of metal nanoparticles obtained from Moringa plant aqueous extract were confirmed by scanning electron microscope (SEM) and X-ray diffraction (XRD), respectively. The amount of antioxidant activity was measured using the DPPH method and the antibacterial properties of Moringa plant aqueous extract and metal nanoparticles obtained from it were measured with the help of disk diffusion and MIC method on two types of bacteria E. coli and S. aureus. The highest amount of antioxidant activity (89.23%) was related to copper nanoparticles at a concentration of 500 μg/ml. As well as E. coli bacteria were more sensitive than S. aureus to the same concentrations of the used treatments and had bigger inhibition zone. Additionally, the diameter of the inhibition zone (in millimeters) was also dependent on the concentration and grew with increasing concentration. The use of copper nanoparticles (especially in higher concentrations) has more antibacterial properties. In terms of survival rate and growth of cultured bacteria using the MIC method, the IC50 of E. coli bacteria compared to S. aureus bacteria in equal concentrations to the applied treatments was low, and the results demonstrated the high sensitivity of E. coli bacteria strain to S. aureus bacteria. According to the obtained results, a significant difference was observed between the aqueous extract obtained from the M. oleifera plant and the metal nanoparticles biosynthesized from it in terms of antioxidant and anticancer activity.


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