Synthesis and Characterization of Gemcitabine-Loaded Magnetic Solid Lipid Nanoparticles: Response Optimization and Modeling via Definitive Screening Designs

Document Type : Original Article


1 Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran

2 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran


Gemcitabine (Gem), as a nucleoside analog, is chemotherapy agent for breast, bladder, and pancreatic cancers. Nanoparticles can cause fewer unwanted side effects by improving the accumulation of drugs in the target tissue. The present study aimed to develop and optimize Gem-MSLNs, gemcitabine-loaded magnetic solid lipid nanoparticles, to minimizing undesired side effects. Gem-MSLNs were synthesized by emulsification and solvent evaporation methods. A definitive screening design was used to optimize the formulation of Gem-MSLNs. Particle size, zeta potential, and the entrapment efficiency of Gem-MSLNs were examined as responses. Characterizing and assessing the optimized Gem-MSLNs was done. The optimal formulation for Gem-MSLNs had a particle size of 96.50 ± 4.36 nm, zeta potential of +72.1 ± 0.3 mV, and an entrapment efficiency of 21.10 ± 2.12%. These results show that the Gem-MSLNs have good colloidal stability in an aqueous environment, which preventing aggregation during the experimentation processes. Gem-MSLNs are hence excellent options for upcoming therapeutic uses.


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