Combination of Hollow Gold Nanoparticles and Aptamer for Accurate and Non-invasive Measurement of Carbamazepine

Shahdoostfard, Faeze; Arhami, Maliheh; Asghari, Alireza; Rajabi, Maryam

doi:10.22075/chem.2025.36384.2330

Combination of Hollow Gold Nanoparticles and Aptamer for Accurate and Non-invasive Measurement of Carbamazepine

Document Type : Original Article

Authors

Faeze Shahdoostfard ¹

Maliheh Arhami ²

Alireza Asghari ²

Maryam Rajabi ²

¹ Department of Chemistry Education, Farhangiyan University, P.O. Box 14665-889, Tehran, Iran

² Department of Chemistry, Semnan University, Semnan, Iran

10.22075/chem.2025.36384.2330

Abstract

In this study, a highly sensitive and accurate aptasensor for carbamazepine detection is proposed based on the combination of hollow gold nanospheres with aptamers. Hollow gold nanospheres with unique advantages are immobilized on the surface of a glassy carbon electrode, and then the aptamer sequence is attached with high density on the surface of the hollow gold nanospheres. By adding carbamazepine to the embedded sensing surface and forming a carbamazepine/aptamer complex, the steric hindrance of the surface is increased and the electron transfer of the electrochemical probe (ferro/ferricyanid) on the surface is limited. Thus, carbamazepine is measured in the range of 1 femtomolar to 900 nanomolar with a detection limit of 3.3333 attomolar. The lack of response in the presence of other species with similar structures or properties, as well as the results of the satisfactory analysis of human biological fluid samples, indicates the very high selectivity of the aptasensor. The proposed strategy for the non-invasive measurement of carbamazepine was found to be in accordance with the principles of green chemistry in terms of two international indicators.

Keywords

Aptasensor

Carbamazepine

Hollow Gold Nanospheres

Non-invasive assay

Green chemistry

Subjects