Optimized Synthesis of Zeolitic Imidazolate Framework-67 and its Application as a Peroxidase-Mimicking Nanozyme for Colorimetric Detection of Hydrogen Peroxide

Document Type : Original Article

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Department of Inorganic Chemistry, Faculty of Chemistry, University of Guilan, Rasht, Iran

Abstract

In recent years, nanostructures with enzyme-like activity, particularly peroxidase mimics, have been regarded as promising alternatives to natural enzymes in a variety of applications. In this study, a cobalt-based zeolitic imidazolate framework-67 ( ZIF - 67 ) was synthesized with high efficiency by exposing an aqueous solution of the reactants to an ammonia atmosphere at ambient temperature. The obtained ZIF- 67 exhibited efficient peroxidase-like activity. Kinetic studies demonstrated that the synthesized ZIF -67 had a stronger binding affinity to o-phenylenediamine ( OPD ) as a substrate, as well as higher reaction rates compared to the enzyme horseradish peroxidase ( HRP ). A ZIF-67-based colorimetric method was further developed for H2O2 detection ( linear response range, 10-4-9.96 × 10-4 M ). This facile and low cost synthesis approach is expected to facilitate the large-scale production and practical application of ZIF - 67 and its derivatives as artificial enzymes in the future.

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Applied Chemistry Today
Volume 20, Issue 76
September 2025
Pages 49-64

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