Identification and Extraction and of Terpenes in Stipacapensis

Document Type : Original Article

Authors

1 Department of Chemistry, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Chemistry Department, faculty of Sciences< Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Chemistry Department, Faculty of sciences, Shahid Chamran university of Ahvaz, Ahvaz, Iran

Abstract

Stipa capensis, the Mediterranean needle-grass, is an annual grass of the genus stipa, in the Poaceae family. It is normal found in the Persian Gulf desert ad semi-desert biome. This plant is indigenous to the Fars, Khuzestan, and west of Iran. In traditional medicine, stipa capensis have been used for illness related to the Nerve system problems and gastric discords. The goal of this study is to identify and characterize the phytochemical compound present in this valuable medicinal plant. This is the first report of the presence of phytochemical in stipa capensis. Stipa capensis is commonly known as Bahman in Iran. The leaves of the plant Stipa capensis was collected from Masjede-Soleiman, Khuzestan, Iran in March, 2015.Basic phytochemical screening from the aerial parts of stipa capensis revealed the presence of terpenoids and steroids. The powdered sample was extracted and was further fractionated successively with n-hexane and methanol.The nonpolar fraction was subjected to column. The separated constituents were subjected to LC-MS and GC-MS study. The spectrum of the unknown compounds was compared with the spectrum of the component stored in the NIST (National Institute Standard and Technology) library.The results pertaining to LC-MS analysis led to the identification of number of terpenes from fraction of the hexane extract of Stipa capensis.This is the first report of the screening and isolation of phytochemical in stipa capensis. This study reports the isolation and identification of terpenoid extracted from stipa capensis on fraction 1and 4 isolated that led to isolation of terpenoid compounds (carotenoids, steroids, cardiac glycosides and prenol lipids.)

Keywords


[1] A. A. Salim, Y.W. Chin, A.D. Kinghorn, Bioactive Molecules and Medicinal Plants Springer (2008).
[2] A. Gurib-Fakim, Molecular Aspects of Medicine, 27 (2006)1.
[3] Kh. Jafarpour, M. Karshenas, Climate-radiation Classification for Iran, 2th climate changes conference, Climatology Center (2000).
[4] M. Karami pour, M. Abedi, Food Novel Technology, 3 (2015) 65.
[5] A. Pengelly, The Constituents of Medicinal Plants. 2th ed, Allen & Unwin (2004).
[6] J. B. Harborne, Phytochemical Methods. 2th ed, chapman and hall (1984).
[7] R. Bekhradi, M. Khayat, Therapeutic Applications of Essential Oils, Morsel press (2006).
[8] W. Ch. Evans, Pharmacognosy, 15th Ed, W.B. Saunders (2002).
[9] S.M. Colegate, R.J. Molyneux, Bioactive Natural Products: Detection, Isolation, and Structural Determination. CRC press (2007).
[10] G. Cao, R. L. Prior, Clinical Chemistry. 44 (1998) 1309.
[11] D. Satyajit, Z. Latif, I. Alexander, Natural Products Isolation. 2th ed. Humana Press Inc (2006).
[12] P. Ghasemi, Afr. J. Tradit Complement Altern Med. 10 (2013) 368.
[13] R.  W. Howarth, "Persian Gulf Desert and Semi-desert." Biomes & Ecosystems, 3 Ipswich,MA: Salem Press, 1000
[14] A.J. Duncan, Animal Behavior. 71 (2006) 93.
[15] C. Harold, American Society for Nutritional Sciences. (2004) 281.
[16] T. John, Carotenoids: Physical, Chemical, and Biological Functions and Properties. Taylor and Francis Group (2010).
[17] C. John, H. Byron, Journal of  Biological Chemistry (1939) 267.
[18] E. Roger, Modern Methods of Pharmaceutical Analysis. CRC Press, 2, (1982).
[19] H. K. Lichtenthaler, C. Buschmann, Current Protocols in Food Analytical Chemistry, (2001) 1.
[20] M. Bowman, Micro Chemical Journal. 56 (1997)10.
[21] S.V. Bhat, B.A. Nagasampagi, M. Sivakumar, Chemistry of Natural Products. Narosa Publishing House, India (2005).
[22] Y. Tanaka, Bulletin of Japanese Society of Scientific Fisheries. 47 (1981) 799.