شیمى کاربردى روز

شیمى کاربردى روز

بررسی فعالیت ضد قارچی و آنتی اکسیدانی نانوامولسیون‌های تهیه شده از اسانس گیاه گندواش

نوع مقاله : مقاله علمی پژوهشی

نویسندگان
1 گروه شیمی آلی، دانشکده شیمی، دانشگاه مازندران، بابلسر، ایران
2 گروه انگل‌شناسی و قارچ‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران
10.22075/chem.2026.40049.2418
چکیده
شوره سر یکی از شایع‌ترین مشکلات پوستی بوده و نقش قارچ Malassezia furfur در بروز آن به‌خوبی شناخته شده است. در این پژوهش، اسانس گیاه گندواش (Artemisia annua) در قالب سه فرمولاسیون نانوامولسیون تهیه و پایداری آن‌ها تحت شرایط مختلف محیطی شامل سانتریفیوژ، گرما، سرما، pHو قدرت یونی ارزیابی شد. سپس فعالیت ضد قارچی بر علیه قارچ M. furrur بررسی شد. همچنین فعالیت آنتی اکسیدانی نانوامولسیون‌ها با اسانس مقایسه شد. ارزیابی پایداری سه فرمولاسیون نانوامولسیون اسانس گندواش نشان داد که نانوامولسیون شماره 1 و ۲ در آزمون‌های پایداری یونی، حرارتی، تغییر pH و سانتریفیوژ کمترین میزان جذب و کدورت را داشت و نسبت به نانوامولسیون شماره 3 پایدارتر بودند. نانو ۳ نیز بیشترین ناپایداری را در تمام آزمون‌ها نشان داد. نتایج آزمون آنتی‌اکسیدانی نشان داد نانوامولسیون 3 با IC50 برابر با 0001/0±2636/1 میلی گرم بر میلی لیتر قدرت آنتی اکسیدانی بیشتری نسبت به اسانس با IC50 برابر با 0001/0±0589/9 میلی گرم بر میلی لیتر داشت. از طرفی تنها نانوامولسیون 2 توانست رشد M. furfur را مهار کند و MIC آن به‌طور قابل‌توجهی کمتر از اسانس خالص بود؛ امری که نقش نانوذرات امولسیونی در بهبود رهایش و اثربخشی ترکیبات فعال را تأیید می‌کند. در مجموع، این مطالعه نشان می‌دهد که نانوامولسیون بهینه‌ شده اسانس گندواش می‌تواند یک گزینه طبیعی و مؤثر برای کنترل عوامل قارچی مرتبط با شوره سر باشد و ظرفیت قابل‌توجهی برای توسعه فرمولاسیون‌های درمانی گیاهی دارد.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Investigation of antifungal and antioxidant activity of nanoemulsions prepared from essential oil of the Gandevash

نویسندگان English

Freshteh Rezazadeh 1
Roya Moghimi 1
Mahtab Ashrafi-khozani 2
1 Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
2 Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
چکیده English

Dandruff is one of the most common skin problems and the role of Malassezia furfur in its occurrence is well known. In this study, Artemisia annua (Gandevash) essential oil was prepared in the form of three nanoemulsion formulations and their stability was evaluated under different environmental conditions including centrifugation, heat, cold, pH and ionic strength. Then, the antifungal activity against M. furfur was investigated. Also, the antioxidant activity of the nanoemulsions was compared with the essential oil. Stability evaluation of the three nanoemulsion formulations of Artemisia annua essential oil showed that nanoemulsion number 1 and 2 had the lowest absorption and turbidity in ionic, thermal, pH change and centrifugation stability tests and was more stable than the nanoemulsion number3. Nano 3 also showed the highest instability in all tests. The antioxidant test results showed that nanoemulsion 3 with IC50 equal to 1.2636±0.0001 mg/ml had greater antioxidant power than the essential oil with IC50 equal to 9.0589±0.0001 mg/ml. On the other hand, only nanoemulsion 2 was able to inhibit the growth of M. furfur and its MIC was significantly lower than that of the pure essential oil; which confirms the role of emulsion nanoparticles in improving the release and effectiveness of active compounds. Overall, this study shows that the optimized nanoemulsion of gandevash essential oil can be a natural and effective option for controlling fungal agents associated with dandruff and has significant potential for the development of herbal therapeutic formulations

کلیدواژه‌ها English

Gandevash
Persian gum
nanoemulsion
Malassezia fungus
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