استفاده از آزمایش طراحی RSM برای تهیه نانو ذرات حاوی پیرازینامید، اتامبوتول و ریفامپین

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 ایران، تهران، دانشگاه آزاد اسلامی واحد تهران شمال، گروه شیمی

2 ایران، سمنان، دانشگاه سمنان، دانشکده دامپزشکی، بخش بیوشیمی

چکیده

در این تحقیق، نانوذرات سه جزئی آهن، نیکل و لیتیوم با دو روش ترکیبی (هم رسوبی، هیدروترمال) و پچینی سل-ژل سنتز شدند. شرایط مطلوب برای تولید MNPs با استفاده از روش هم رسوبی در pH=13 با 1000 دور در دقیقه و در دمای 72 درجه سانتیگراد در محدوده ی نیتروژن به دست آمد. بهترین شرایط برای تهیه نانو ذرات با استفاده از روش پچینی سل-ژل شامل حل نمک های نیترات سه فلز در آب و سپس اضافه کردن اسید سیتریک و اتیلن گلیکول به ترتیب در دماهای 60 و 80 با کنترل pH = 7 و قرار دادن نمونه ها در دمای 190 و 500 برای 720 و 60 دقیقه بدست آمد. در مقایسه دو روش، مورفولوژی و اندازه نانو ذرات مغناطیسی تولید شده با روش ترکیبی هم رسوبی - هیدروترمال مناسب تر بود. برای بررسی بهترین نانوذرات سنتز شده از روش بررسی سطوح پاسخ (RSM) در اندازه گیری استفاده شد. گروه های سیلیس و آمین در نانوذرات پوشش داده شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Using the design experiment RSM for the preparation of nanoparticles containing pyrazinamide, ethambutol and rifampin

نویسندگان [English]

  • Fariba Tadayon 1
  • reza jamshidi 2
  • parisa Jamshidpoor 1
1 Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 department of biochemistry, faculty of veterinary, semnan university, Semnan, Iran
چکیده [English]

In this study, three-component nanoparticles of iron, nickel and lithium were synthesized with two methods of combined technique (co-precipitation, hydrothermal) and pechini sol-gel. The optimum conditions for producing magnetic nanoparticles (MNPs) by co-precipitation method were found to be at pH=13 with constant stirring rate of 1000 rpm and at temperature of 72 ˚C under nitrogen atmosphere. The situation for preparing nanoparticles by pechini sol-gel method including dissolving nitrate salt of three metals in water then adding citric acid and ethylene glycol respectively in temperatures 60 and 80 0 C with pH=7 and placing sample in temperatures 190 and 500 0 C for 720 and 60 minutes respectively. In the comparison of two methods, morphology and size of produced magnetic nanoparticles with combined method of co-precipitation- hydrothermal was more suitable. Response surface methodology was used to verify the best synthesized nanoparticles in size. The surface of MNPs was modified with silica and amine groups to develop a suitable drug delivery system.

کلیدواژه‌ها [English]

  • magnetic nanoparticles
  • Response surface methodology
  • Pechini sol-gel
  • Hydrothermal

مراجع

[1] M. Ferrari, Cancer nanotechnology: Opportunities and challenges, Nature Reviews. Cancer 5 (2005) 161.
[2] N. Sanvicens, M.P. Marco, Multifunctional nanoparticles - properties and prospects for their use in human medicine, Trends in Biotechnology 26 (2008) 425.
[3] O.C. Farokhzad, R. Langer, Nanomedicine: Developing smarter therapeutic and diagnostic modalities, Advanced Drug Delivery Reviews 58 (2006) 1456.
[4] J. Dobson, Magnetic nanoparticles for drug delivery, Drug Dev. Res. 67 (2006) 55.
[5] Sun, Conroy, Jerry SH Lee, and Miqin Zhang. "Magnetic nanoparticles in MR imaging and drug delivery." Advanced drug delivery reviews 60 (2008) 1252.
[6] O. Veiseh, W.Jonathan, and M. Zhang. "Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging." Advanced drug delivery reviews 62.3 (2010) 284.
[7] J.Andreas, et al. "Inductive heating of ferromagnetic particles and magnetic fluids: physical evaluation of their potential for hyperthermia." International Journal of Hyperthermia 25.7 (2009) 499.
[8] T. Nhiem, and Thomas J. Webster. "Magnetic nanoparticles: biomedical applications and challenges." Journal of Materials Chemistry 20.40 (2010): 8760.
[9] Y. Fang, et al. "Superparamagnetic iron oxide nanoparticle-embedded encapsulated microbubbles as dual contrast agents of magnetic resonance and ultrasound imaging." Biomaterials 30.23 (2009): 3882.
[10] C.Miriam, et al. "Biological applications of magnetic nanoparticles." Chemical Society Reviews 41.11 (2012) 4306.
[11] Q. A. Pankhurst, et al. "Progress in applications of magnetic nanoparticles in biomedicine." Journal of Physics D: Applied Physics 42.22 (2009): 224001.
[12]  W. Jiang, H. Yang, S. Yang, H. Horng, J. Hung, Y. Chen, C.-Y. Hong, Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible, J. of Mag. and Mag. Mat. 283 (2) (2004) 210.
[13] V. Pramod Kumar, et al. "Superparamagnetic fluorescent nickel–enzyme nanobioconjugates: synthesis and characterization of a novel multifunctional biological probe." Journal of Materials Chemistry 20.18 (2010): 3722.
[14]  A. Tadayon, R. Jamshidi, A. Esmaeili, International Journal of Pharmaceutics, Delivery of tissue plasminogen activator and streptokinase magnetic nanoparticles to target vascular diseases,( November 2015) 428.
[15] S.PoornimaBudime, and N. PoklarUlrih. "Multifunctional superparamagnetic iron oxide nanoparticles: promising tools in cancer theranostics." Cancer letters 36.1 (2013) 8.
[16] TK. Thanh, Nguyen, and Luke AW Green. "Functionalization of nanoparticles for biomedical applications." Nano Today 5.3 (2010) 213.
[17] H. Rui, et al. "Synthesis, functionalization, and biomedical applications of multifunctional magnetic nanoparticles." Advanced Materials 22.25 (2010) 2729.
[18] B.J. Boyd, Past and future evolution in colloidal drug delivery systems, Expert Opinion on Drug Delivery 5 (2008) 69.
[19] V.P. Torchilin, Multifunctional nanocarriers, Advanced Drug Delivery Reviews 58 (2006) 1532.
[20] E. Allemann, R. Gurny, E. Doelker, Drug-loaded nanoparticles: preparation methods and drug targeting issues, Eur. J. of Pharm. and Biopharm. 39 (1993) 173.
[21] N. Kohler, Fryxell GE, Zhang M. A bifunctional poly (ethylene glycol) silane immobilized on metallic oxide-based nanoparticles for conjugation with cell targeting agents. J Am Chem Soc. 126 (2004) 1267206.
[22] A.H. Abdullah, H.J. Melanie Moey and N.  Azah, Response surface methodology analysis of the photocatalytic removal of Methylene Blue using bismuth vanadate prepared via polyol route. J Environ Sci .24 (2012).1694.
 [23] CQ. Yang, G. Wang G, et al. Effect of ultrasonic treatment on dispensability of Fe3O4 nanoparticles and synthesis of multi-core Fe3O4/SiO2 core/shell nanoparticles. J Mater Chem.15 (2005) 4252.
شیمى کاربردى روز
دوره 13، شماره 49
دی 1397
صفحه 53-64

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