1 گروه زیست شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، ایران
2 گروه شیمی آلی، دانشکده شیمی، دانشگاه مازندران، بابلسر، ایران
عنوان مقاله [English]
In this study antioxidant properties and total phenolic content of the extracts of Nelumbo nucifera seeds were examined. The extracts were prepared using the solvents water, methanol, water : methanol (1:1), ethyl acetate, acetone and chloroform. Two experimental methods including DPPH (1,1-Diphenyl-2-picryl-hydrazyl) radical scavenging activity and total antioxidant capacity were used for characterization of antioxidant activity of the extracts. The six extracts showed varying degrees of efficacy in each assay in a dose-dependent manner. The acetone extract with the highest amount of total phenolic content (20.12 Â± 0.02 g GAE/100 g dried extract), was the most potent antioxidant in both assays used. The LSA exhibited strong free radical scavenging activity as evidenced by the low EC50 values in DPPH assay (4.28 Î¼g/ml) and in total antioxidant assay by maximum activity (25% of ascorbic acid). On the basis of the results, the acetone extract of Nelumbo nucifera seeds were found to serve as a potential source of natural antioxidants.