شیمى کاربردى روز

شیمى کاربردى روز

سنتز و شناسایی کمپلکس سیکلومتاله پالادیوم (II) حاوی لیگاند تیروزین: مطالعات تجربی و نظری برهمکنش با ماکرومولکول های زیستی

نوع مقاله : مقاله علمی پژوهشی

نویسندگان
1 دانشکده شیمی. دانشگاه خوارزمی. تهران. ایران
2 مرکز تحقیقات بیوشیمی و بیوفیزیک، دانشگاه تهران، تهران، ایران
چکیده
با توجه به اهمیت لیگاندهای زیست فعال در طراحی و ساخت ترکیبات با ویژگی ضدتوموری، کمپلکس تک هسته ای C^C- سیکلومتاله پالادیوم (II) فسفر ایلید حاوی آمینواسید تیروزین سنتز شد و با استفاده از روش های طیف سنجی مادون قرمز (FT-IR) و رزونانس مغناطیسی هسته (NMR) مورد شناسایی قرارگرفت. برهمکنش کمپلکس تک هسته ای سنتز شده با ماکرومولکول های حیاتی دئوکسی ریبونوکلئیک اسید (DNA) و سرم آلبومین گاوی (BSA)، بوسیله تکنیک های تجربی طیف‌سنجی جذب مرئی- فرابنفش (UV-Vis) و طیف‌سنجی نشر فلوئورسانس مورد مطالعه قرار گرفت. مطالعات تجربی نشان دهنده برهمکنش از نوع اینترکلیشن میان کمپلکس و DNA است. نتایج حاصل از روش های طیف‌سنجی جذب مرئی- فرابنفش و نشر فلوئورسانس نشان دهنده برهمکنش قابل توجه میان کمپلکس و پروتئین BSA است. همچنین، طبق نتایج حاصل از محاسبات داکینگ مولکولی، کمپلکس به جایگاه های آب گریز پروتئین BSA اتصال یافته است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Synthesis and characterization of cyclometalated Pd(II) complex bearing tyrosine: Experimental and theoretical study of interaction with biomacromolecules

نویسندگان English

Sedigheh Abedanzadeh 1
Mahsa Khaghani Azar 1
Bagher Davaeil 2
1 Faculty of Chemistry. Kharazmi University. Tehran. Iran
2 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
چکیده English

ABSTRACT
Considering the important role of bioactive ligands in designing antitumor complexes, mononuclear C^C-cyclometalated Pd (II) complex containing amino acid, tyrosine, was synthesized and identified using Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic methods. The interaction of the synthesized mononuclear complex with the biomacromolecules, deoxyribonucleic acid (DNA) and bovine serum albumin (BSA), was studied by UV-Vis absorption and fluorescence emission. Based on the experimental studies, an intercalating mode was proposed for the interaction between the complex and DNA. The UV-Vis absorption and fluorescence emission spectroscopies indicated the high affinity of the complex for BSA binding. Molecular docking calculations revealed that the hydrophobic interactions play important roles in complex – BSA binding.

کلیدواژه‌ها English

Palladacycle
Aminoacid
DNA
BSA
Molecular docking

This is an open access article under the CC-BY-SA 4.0 license.( https://creativecommons.org/licenses/by-sa/4.0/)

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