سنتز سبز نانوذرات فلزی با استفاده از عصاره گیاه مورینگا (Moringa oleifera L.) و بررسی خواص ضدباکتری و آنتی‌اکسیدانی

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 دانشکده کشاورزی و منابع طبیعی، گروه تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشکده داروسازی، گروه فارماسیوتیکس، دانشگاه علوم پزشکی‌، واحد اردبیل، اردبیل، ایران

3 دانشکده داروسازی، گروه فارماکوگنوزی‌، دانشگاه علوم پزشکی‌، واحد اردبیل، اردبیل، ایران

چکیده

گیاه مورینگا با نام علمی‌ Moringa oleifera L. دارای ترکیبات شیمیایی مهم دارویی از جمله فلاونوئیدها‌ است که دارای خواص آنتی‌اکسیدانی و ضدسرطانی می‌باشد. در این پژوهش ویژگی‌های نانوذرات فلزی همچون آهن، مس، روی و نقره بیوسنتز شده از عصاره آبی گیاه M.‌ oleifera مورد مطالعه قرار گرفته و در ادامه به خواص آنتی‌اکسیدانی و ضدباکتریایی عصاره آبی حاصل از اندام هوایی این گیاه و نانوذرات فلزی حاصل از آن پرداخته شد. اندازه و ساختار نانوذرات فلزی حاصل از عصاره آبی گیاه مورینگا به ترتیب به کمک میکروسکوپ الکترونی روبشی (‌SEM) و پراش اشعه ایکس (XRD) تأیید شد. میزان فعالیت آنتی‌اکسیدانی به کمک روش DPPH و خاصیت ضدباکتریایی عصاره‌آبی گیاه مورینگا و نانوذرات فلزی حاصل از آن به کمک روش دیسک دیفیوژن و MIC بر روی دو گونه باکتری E. coli و S. aureus سنجیده شد. طبق نتایج به دست آمده بیشترین میزان فعالیت آنتی-اکسیدانی (23/89 درصد) مربوط به نانوذره مس در غلظت 500 میکروگرم در میلی‌لیتر بود. هم‌چنین باکتری E. coli نسبت به S. aureus نسبت به غلظت‌های برابر تیمارهای مورد استفاده حساس‌تر بوده و هاله‌های بزرگ‌تری را ایجاد کردند. از نظر میزان زنده‌مانی باکتری‌های کشت شده به کمک روش MIC نیز میزان IC50 باکتری E. coli نسبت به باکتری S. aureus در غلظت‌های برابر تیمارهای اعمال شده پایین‌تر بوده و بیانگر حساسیت بالای سویه باکتری E. coli نسبت به باکتری S. aureus بود. بین عصاره آبی حاصل از گیاه M. oleifera و نانوذرات فلزی بیوسنتز شده از آن از نظر میزان فعالیت آنتی-اکسیدانی و ضدسرطانی تفاوت معنی‌داری داشت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Green synthesis of metal nanoparticles using aqueous extract of Moringa oleifera L. and investigating their antioxidant and antibacterial properties

نویسندگان [English]

  • Mehran Noruzpuor 1
  • Rasool Asghari Zakaria 1
  • Nasser Zare 1
  • Hossein Ali Ebrahimi 2
  • Hamed Parsa 3
  • Shima Bourang 1
1 Faculty of Agriculture and Natural Resources, Department of Plant Production and Genetics, Mohaghegh Ardabili University, Ardabil, Iran
2 Faculty of Pharmacy, Department of Pharmaceutics, University of Medical Sciences, Ardabil Branch, Ardabil, Iran
3 Faculty of Pharmacy, Department of Pharmacognosy, University of Medical Sciences, Ardabil Branch, Ardabil, Iran
چکیده [English]

Moringa plant with the scientific name Moringa oleifera L. has important medicinal chemical compounds including flavonoids, which have antioxidant and anticancer properties. In this research, the properties of metal nanoparticles such as Iron, Copper, Zinc and Silver biosynthesized from the aqueous extract of M. oleifera plant have been studied, and then the antioxidant and antibacterial properties of the aqueous extract obtained from the aerial parts of this plant and the nanoparticles have been studied. The resulting metal was treated. The size and structure of metal nanoparticles obtained from Moringa plant aqueous extract were confirmed by scanning electron microscope (SEM) and X-ray diffraction (XRD), respectively. The amount of antioxidant activity was measured using the DPPH method and the antibacterial properties of Moringa plant aqueous extract and metal nanoparticles obtained from it were measured with the help of disk diffusion and MIC method on two types of bacteria E. coli and S. aureus. The highest amount of antioxidant activity (89.23%) was related to copper nanoparticles at a concentration of 500 μg/ml. As well as E. coli bacteria were more sensitive than S. aureus to the same concentrations of the used treatments and had bigger inhibition zone. Additionally, the diameter of the inhibition zone (in millimeters) was also dependent on the concentration and grew with increasing concentration. The use of copper nanoparticles (especially in higher concentrations) has more antibacterial properties. In terms of survival rate and growth of cultured bacteria using the MIC method, the IC50 of E. coli bacteria compared to S. aureus bacteria in equal concentrations to the applied treatments was low, and the results demonstrated the high sensitivity of E. coli bacteria strain to S. aureus bacteria. According to the obtained results, a significant difference was observed between the aqueous extract obtained from the M. oleifera plant and the metal nanoparticles biosynthesized from it in terms of antioxidant and anticancer activity.

کلیدواژه‌ها [English]

  • Antibacterial properties
  • Metallic nanoparticles
  • Moringa oleifera
  • green synthesis

مراجع

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شیمى کاربردى روز
دوره 19، شماره 71
تیر 1403
صفحه 283-302

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