سنتز سبز و مشخصه‌یابی نانو حسگرهای نقاط کربنی با استفاده از پوست تخمه کدو جهت اندازه‌گیری اسپکتروفلوریمتری ال- سیستئین

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

گروه شیمی، دانشکده علوم پایه، دانشگاه ولی عصر(عج) رفسنجان، رفسنجان، ایران

چکیده

در این تحقیق نقاط کربنی با شدت نورتابی بسیار بالا با استفاده از پوست تخمه کدو به عنوان یک منبع جدید و سبز توسط روش آب گرمایی سنتز شد. نقاط کربنی تهیه شده توسط آنالیز پراش اشعه ایکس، طیف‌سنجی تبدیل فوریه فروسرخ و آنالیز میکروسکوپی الکترونی روبشی مشخصه یابی شدند. متوسط اندازه ذرات نقاط کربنی سنتز شده حدود 59 نانومتر برآورد گردید. علاوه بر این ویژگی نورتابی نقاط کربنی سنتز شده نیز با استفاده از روش فوتولومینسانس بررسی گردید و با توجه به شدت نورتابی بالایی که در طول موج تحریک 340 نانومتر و طول موج نشری 425 نانومتر نشان دادند، از آنها به عنوان نانو حسگر برای اندازه‌گیری ال- سیستئین استفاده شد. با افزودن ال- سیستئین میزان نشر محلول نقاط کربنی کاهش یافت. جهت افزایش حساسیت روش پارامترهای موثر بر شدت خاموش‌کنندگی ال- سیستئین مانند pH، زمان برهمکنش و قدرت یونی محیط مورد ارزیابی و بهینه سازی قرار گرفت.در شرایط بهینه آزمایشگاهی، گستره خطی 500- 5 میکرومولار، حدتشخیص 1 میکرومولار و انحراف استاندارد نسبی (در غلظت 10 میکرومولار از ال- سیستئین) 3 درصد به دست آمد. از روش افزودن برای بررسی صحت روش پیشنهادی استفاده شد که با توجه به درصد‌ بازیابی‌ها ( 102-96 درصد) روش پیشنهادی یک روش قابل اعتماد برای اندازه‌گیری ال- سیستئین در نمونه‌های مورد آزمایش می‌باشد. در نهایت نانوحسگر تهیه شده برای اندازه‌گیری ال- سیستئین در نمونه‌های آب شهر با موفقیت مورد استفاده قرار گرفت.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Green Synthesis and Characterization of Carbon Dots Nanosensors Using Pumpkin Seed Shell for Spectrofluorimetric Determination of L-cysteine

نویسندگان [English]

  • Afsaneh Zarei Manujan
  • Alireza Bazmandegan-shamili
  • Mohammad Sabet
  • Masoud Rohani Moghadam
Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
چکیده [English]

In this research, carbon dots with high luminescence intensity were synthesized using pumpkin seed shell as a new and green source with hydrothermal method. The prepared carbon dots were characterized using X-ray diffraction, Fourier transform infrared spectroscopy and Scanning electron microscopy. The average size of the synthesized carbon dots was estimated about 59 nm. In addition, the luminescence property of the synthesized carbon dots was evaluated using photoluminescence analysis and according to their high luminescence property at excitation wavelength of 340 nm and emission wavelength of 425 nm, they were used as nanosensor for the determination of L-cysteine. By addition of L-cysteine, the emission intensity of carbon dots solution was reduced. In order to enhancement of the method sensitivity, the effective parameters on the quenching intensity of L-cysteine such as pH, interaction time and environment ionic strength were examined and optimized. Under the laboratory optimum conditions, linear range of 5-500 µM, limit of detection of 1.0 µM and relative standard deviation (at 10 µM of L-cysteine) of 3% was obtained. The accuracy of the proposed method was evaluated using spiking procedure and according to the recoveries percent (96-102), the proposed method is a reliable method for determination of L-cysteine in the examined samples. Finally, the prepared nanosensor was successfully used for the determination of L-cysteine in tap water samples.

کلیدواژه‌ها [English]

  • L-cysteine
  • Luminescence
  • Pumpkin seed shell
  • Carbon dots
  • Nanosensor
[1] Yin, Z., Chen, S., Xu, Z., Zhang, C., He, J., Zou, J., chen, D., & sun, W. (2020).Flotation separation of molybdenite from chalcopyrite using an environmentally-efficient depressant L-cysteine and its adsoption mechanism. Minerals Engineering, 156, 106438.
[2] Clemente Plaza, N., Reig García-Galbis, M., & Martínez-Espinosa, RM. (2018). Effects of the Usage of l-Cysteine (l-Cys) on Human Health. Molecules, 23(3), 575.
[3] Selçuk K., Kivrak, H., & Aktaş N. (2021). Novel CNT supported molybdenum catalyst for detection of L-cysteine in its natural environment. Catalysts, 11(12), 1561.
[4] Juliano, C., Cossu, M., Rota, MT., Satta, D., Poggi, P., & Giunchedi, P.  (2011). Buccal tablets containing cysteine and chlorhexidine for the reduction of acetaldehyde levels in the oral cavity. Drug development and industrial pharmacy, 37(10) 1192-1199.
[5] Dong, W., Wang, R., Gong, X., Liang, W., & Dong, C. (2019). A far-red FRET fluorescent probe for ratiometric detection of l-cysteine based on carbon dots and N-acetyl-l-cysteine-capped gold nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 213, 90-96.
[6] Fernandéz, J., Rouzard, K., Voronkov, M., Huber, K., Stock, J., Stock, M., gordon, j.s., & perez, E.(2015). Anti‐inflammatory and anti‐bacterial properties of tetramethylhexadecenyl succinyl cysteine (TSC): a skin‐protecting cosmetic functional ingredient. International Journal of Cosmetic Science, 37(1),129-133.
[7] Liang, Y-C., Zhao, Q., Wu, X-Y., Li, Z., Lu, Y-J., Liu, Q., dong, L., & shan, C-X. (2019). A ratiometric fluorescent nanoprobe based on quenched carbon dots-rhodamine B for selective detection of L-cysteine. Journal of Alloys and Compounds, 788, 615-622.
[8] Hsu, CN., Lin, YJ., Lu, PC., & Tain, YL. (2018). Early supplementation of d‐cysteine or L‐cysteine prevents hypertension and kidney damage in spontaneously hypertensive rats exposed to high‐salt intake. Molecular Nutrition & Food Research, 62(2), 1700596.
[9] Xu, H., Huang, S., Liao, C., Li, Y., Zheng, B., Du, J., Xiao, D. (2015). Highly selective and sensitive fluorescence probe based on thymine-modified carbon dots for Hg2+ and L-cysteine detection. RSC advances, 5(108), 89121-89127
[10] Wu, H., Jiang, J., Gu, X., & Tong, C. (2017).Nitrogen and sulfur co-doped carbon quantum dots for highly selective and sensitive fluorescent detection of Fe (III) ions and L-cysteine. Microchimica Acta, 184, 2291-2298.
[11] Zeng, X., Zhang, X., Zhu, B., Jia, H., & Li, Y.(2012). A highly selective wavelength-ratiometric and colorimetric probe for cysteine. Dyes and Pigments, 94(1), 10-15.
[12] Lee, SA., Lee, JJ., Shin, JW., Min, KS., & Kim C. (2015). A colorimetric chemosensor for the sequential detection of copper(II) and cysteine. Dyes and Pigments, 116, 131-138.
[13] Lau, C., Qin, X., Liang, J., & Lu, J. (2004).Determination of cysteine in a pharmaceutical formulation by flow injection analysis with a chemiluminescence detector. Analytica Chimica Acta, 514(1), 45-49.
[14] Wada, M., Kuroki, M., Minami, Y., Ikeda, R., Sekitani, Y., Takamura, N., Kawakami, S., Kuroda, N., & Nakashima, K. (2014). Quantitation of sulfur-containing amino acids, homocysteine, methionine and cysteine in dried blood spot from newborn baby by HPLC-fluorescence detection. Biomedical Chromatography, 28(6), 810-814.
[15] Vellasco, AP., Haddad, R., Eberlin, MN., & Höehr, NF. (2002). Combined cysteine and homocysteine quantitation in plasma by trap and release membrane introduction mass spectrometry. Analyst, 127(8), 1050-1053.
[16] Jin, W., & Wang, Y. (1997). Determination of cysteine by capillary zone electrophoresis with end-column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation. Journal of Chromatography A, 769(2),307-314.
[17] Chaichi, MJ., Ehsani, M., Khajvand, T., Golchoubian, H., & Rezaee, E. (2014). Determination of cysteine and glutathione based on the inhibition of the dinuclear Cu (II)-catalyzed luminol–H2O2 chemiluminescence reaction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ,122, 405-410.
[18] Devasenathipathy, R., Mani ,V., Chen, S-M., Kohilarani, K., & Ramaraj, S. (2015). Determination of L-cysteine at iron tetrasulfonated phthalocyanine decorated multiwalled carbon nanotubes film modified electrode. International Journal of Electrochemical Science, 10(1),682-690.
[19] Yan, L., Kong, Z., Shen, W., Du, W., Zhou, Y., & Qi, Z. (2016) A label-free turn-on fluorescence probe for rapidly distinguishing cysteine over glutathione in water solution. Analytical Biochemistry, 500, 1-5.
[20] Huang, S., Wang, L., Huang, C., Hu, B., Su, W., & Xiao, Q. (2016). Graphene quantum dot coupled with gold nanoparticle based “off-on” fluorescent probe for sensitive and selective detection of L-cysteine. Microchimica Acta, 183,1855-1864.
[21] Afshani, J., Badiei, A., Karimi, M., Lashgari, N., & Ziarani, GM. (2016). A single fluorescent sensor for Hg2+ and discriminately detection of Cr3+ and Cr(VI). Journal of Fluorescence, 26(1), 263-270.
[22] Soltani, B., Hosseini sadr, M., & karampour, S. (2020). Phenolate-based ligands and their complexes with samarium and praseodymium: Synthesis, characterization and investigation of fluorescence properties. Applied Chemistry Today. (in persion)
[23] Manzoori, J L., Niaei, N., & Abulhassani, J. (2015). Spectrofluorimetric determination of daunorubicin using terbium-deferasirox as a fluorescence probe. Applied Chemistry Today, 9 (32), 67-74.
[24] Abbasi, P., & Shafaatian, B. (2020). Synthesis, characterization, fluorescence and electrochemical studies of new ferrocene Schiff base ligand containing nitrogen donor atoms and its palladium(II), nickel(II) and copper(II) complexes. Applied Chemistry Today, 15(55), 111-1124. (in persion)
[25] Azadbakht, R., Almasi, T., & Khanabadi, J. (2016). A new fluorescent chemosensor for detection of aluminium ions. Applied Chemistry Today, 11(38), 75-84. (in persion)
[26] Cui, L., Ren, X., Sun, M., Liu, H., & Xia, L. (2021). Carbon dots: Synthesis, properties and applications. Nanomaterials, 11(12), 3419.
[27] Tabaraki ,R., Sadeghi nezhad, N., & Yousefi poor, S. (2018). Green fluorescent sensor based on carbon quantum dots for Cr(VI) determination. Applied Chemistry Today, 13(48), 339-50. (in persion)
[28] Hu, L., Sun, Y., Li, S., Wang, X., Hu, K., Wang, L., Liang, X-J., & Wu, Y. (2014). Multifunctional carbon dots with high quantum yield for imaging and gene delivery. Carbon, 67, 508-513.
[29] De, B., & Karak, N. (2017). Recent progress in carbon dot–metal based nanohybrids for photochemical and electrochemical applications. Journal of Materials Chemistry A, 5(5), 1826-1859.
[30] Huang, Q., Bao, Q., Wu, C., Hu, M., Chen, Y., Wang, L., & Chen, W. (2022). Carbon dots derived from Poria cocos polysaccharide as an effective “on-off” fluorescence sensor for chromium (VI) detection. Journal of Pharmaceutical Analysis, 12(1), 104-112.
[31] Atchudan, R., Edison, TNJI., Perumal, S., Vinodh, R., & Lee, YR. (2019). Betel-derived nitrogen-doped multicolor carbon dots for environmental and biological applications. Journal of Molecular Liquids, 296, 111817.
[32] Cao, M., Xia, C., Xia, J., Jiang, D., Yu, C., & Li, H. (2019). A yellow carbon dots-based phosphor with high efficiency for white light-emitting devices. Journal of Luminescence, 206, 97-104.
[33] Ahn, J., Song, Y., Kwon,  JE., Lee, SH., Park, KS., Kim, S., Woo, j., Kim, H.(2019). Food waste-driven N-doped carbon dots: Applications for Fe3+ sensing and cell imaging. Materials Science and Engineering: C, 102, 106-112.
[34] Wang, L., & Zhou, HS. (2014). Green synthesis of luminescent nitrogen-doped carbon dots from milk and its imaging application. Analytical Chemistry, 86(18), 8902-8905.
[35] Aji, MP., Susanto., Wiguna, PA., & Sulhadi. (2017).Facile synthesis of luminescent carbon dots from mangosteen peel by pyrolysis method. Journal of Theoretical and Applied Physics, 11(2), 119-126.
[36] Thongsai, N., Tanawannapong, N.,  Praneerad, J., Kladsomboon, S., Jaiyong, P., & Paoprasert, P. (2019). Real-time detection of alcohol vapors and volatile organic compounds via optical electronic nose using carbon dots prepared from rice husk and density functional theory calculation. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 560, 278-287.
[37] D, P., Singh, L., Thakur, A., & Kumar, P. (2019). Green synthesis of glowing carbon dots from Carica papaya waste pulp and their application as a label-freechemo probe for chromium detection in water. Sensors and Actuators B: Chemical, 283, 363-372.
[38] Wang, C., Lan, Y., Yuan, F., Fereja, TH., Lou, B., Han, S., Li, J., & Xu, G. (2020). Chemiluminescent determination of L-cysteine with the lucigenin-carbon dot system. Microchimica Acta, 187(50), 1-6.
[39] Bamdad, F., Khorram, F., Samet, M., Bamdad, K., Sangi, MR., & Allahbakhshi, F. (2016). Spectrophotometric determination of L-cysteine by using polyvinylpyrrolidone-stabilized silver nanoparticles in the presence of barium ions. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 161, 52-57.
[40] Vieira, IdC., & Fatibello-Filho, O. (1999). L-Cysteine determination using a polyphenol oxidase-based inhibition flow injection procedure. Analytica Chimica Acta, 399(3), 287-293.
[41] Raoof, J-B., Ojani, R., & Beitollahi, H. (2007). L-Cysteine voltammetry at a carbon paste electrode bulk-modified with ferrocenedicarboxylic acid. Electroanalysis, 2007, 19(17), 1822-1830.