اندازه گیری ولتامتری داروی اسیکلوویر با استفاده از الکترود گلسی کربن نانو متخلخل

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

گروه شیمی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران

چکیده

در این پروژه ی تحقیقاتی، یک روش ساده الکتروشیمیایی با تبدیل آسان سطح الکترود به ساختاری در ابعاد نانو و متخلخل ارائه شد. نانومتخلل در سطح الکترود کربن شیشه ای به روش کرنوآمپرومتری با اعمال پتانسیل ترسیب داده شد. الکترود پیشنهادی جهت اندازه گیری داروی آسیکلویر استفاده شد. تحت شرایط بهینه، بهبود قابل توجهی در رفتار الکتروشیمیایی آسیکلویر در سطح الکترود اصلاح شده نسبت به الکترود اصلاح نشده مشاهده شد. حد تشخیص 13/0 میکرومولار و رنج خطی 99/1 تا 6/19 میکرومولار برای تعیین آسیکلویر در سطح اصلاح شده با استفاده از روش ولتامتری پالس تفاضلی در بافر فسفات به دست آمد. نتایج رضایت بخشی در تعیین آسیکویر با الکترود اصلاح شده در نمونه های سرم خون به دست آمد

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Voltammetric determination of acyclovir using nanoporous glassy carbon electrode

نویسندگان [English]

  • Ghasem Karim-Nezhad
  • Zeynab Khorablou
Department of Chemistry, Payame Noor University, P.O. BOX 19395-3697 Tehran, IRAN
چکیده [English]

In this research project, a simple electrochemical method was presented with the easy conversion of the electrode surface into a nano-sized and porous structure. The nanoporous was deposited on the surface of glassy carbon electrode by chronoamperometric method by applying potential. The proposed electrode was used to measure acyclovir drug. Under optimal conditions a significant improvement in the electrochemical behavior of acyclovir was observed on the surface of the modified electrode compared to the unmodified electrode. The detection limit of 0.13 μM and the linear range of 1.99 to 19.6 μM were obtained for the determination of acyclovir at the surface of modified electrode using differential pulse voltammetric method in phosphate buffer. Satisfactory results were obtained in the determination of acyclovir with the modified electrode in blood serum samples.

کلیدواژه‌ها [English]

  • Voltammetry
  • Glassy carbon electrode
  • Nano porous
  • Acyclovir

This is an open access article under the CC-BY-SA 4.0 license.( https://creativecommons.org/licenses/by-sa/4.0/)

[1] Wagstaff, A.J., Faulds, D., Goa, K.L. (1994) Aciclovir. A reappraisal of its antiviral activity, pharmacokinetic properties and therapeutic efficacy. Drugs 47(1) 153-205.
[2] Hosseinzadeh, H. (1390). Potential drug delivery systems from chitosan-g-poly(sodium acrylate-co-acrylamide) superabsorbent hydrogels. Applied Chemistry 6(21), 21-33. in Persian
[3] Lu, Y., Celum, C., Wald, A., Baeten, J. M., Cowan, F., Delany-Moretlwe, S., Hendrix, C. W. (2012). Acyclovir achieves a lower concentration in African HIV-seronegative, herpes simplex virus 2-seropositive women than in non-African populations. Antimicrobial Agents and Chemotherapy, 56(5), 2777–2779.
[4] Adair, J. C., Gold, M., & Bond, R. E. (1994). Acyclovir neurotoxicity: clinical experience and review of the literature. Southern Medical Journal, 87(12), 1227–1231.
[5] Chiou, W.L., & Barve, A. (1998). Linear correlation of the fraction of oral dose absorbed of 64 drugs between humans and rats. Pharm. Res., 15(11) 1792-1795.
[6] Ayad, M. M., Abdellatef, H. E., El-Henawee, M. M., & El-Sayed, H. M. (2007). Spectrophotometric and spectrofluorimetric methods for analysis of acyclovir and acebutolol hydrochloride. Spectrochim. Acta A, 66(1) 106-110.
[7] Yu, L., & Xiang, B. (2008). Quantitative determination of acyclovir in plasma by near infrared spectroscopy. Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 90(1), 63–66.
[8] Huidobro, A. L., Rupérez, F. J., & Barbas, C. (2005). LC methods for acyclovir and related impurities determination. Journal of Pharmaceutical and Biomedical Analysis, 37(4), 687–694.
[9] Tzanavaras, P. D., & Themelis, D. G. (2007). High-throughput HPLC assay of acyclovir and its major impurity guanine using a monolithic column and a flow gradient approach. Journal of Pharmaceutical and Biomedical Analysis, 43(4), 1526–1530.
[10] Sasanya, J. J., Abd-Alla, A. M. M., Parker, A. G., & Cannavan, A. (2010). Analysis of the antiviral drugs acyclovir and valacyclovir-hydrochloride in tsetse flies (Glossina pallidipes) using LC-MSMS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(26), 2384–2390.
[11] Wang, F., Chen, L., Chen, X., & Hu, S. (2006). Studies on electrochemical behaviors of acyclovir and its voltammetric determination with nano-structured film electrode. Analytica Chimica Acta, 576(1), 17–22.
[12] Ilager, D., Shetti, N. P., Malladi, R. S., Shetty, N. S., Reddy, K. R., & Aminabhavi, T. M. (2021). Synthesis of Ca-doped ZnO nanoparticles and its application as highly efficient electrochemical sensor for the determination of anti-viral drug, acyclovir. Journal of Molecular Liquids, 322, 114552.
[13] Wang, P., Gan, T., Zhang, J., Luo, J., & Zhang, S. (2013). Polyvinylpyrrolidone-enhanced electrochemical oxidation and detection of acyclovir. Journal of Molecular Liquids, 177, 129–132.
[14] Shahrokhian, S., Azimzadeh, M., & Amini, M. K. (2015). Modification of glassy carbon electrode with a bilayer of multiwalled carbon nanotube/tiron-doped polypyrrole: Application to sensitive voltammetric determination of acyclovir. Materials Science & Engineering. C, Materials for Biological Applications, 53, 134–141.
[15] Joseph, R., & Kumar, K. G. (2011). Electrochemical sensing of acyclovir at a gold electrode modified with 2-mercaptobenzothiazole-[5,10,15,20-tetrakis-(3-methoxy-4-hydroxyphenyl)porphyrinato]copper(II). Analytical Sciences: The International Journal of the Japan Society for Analytical Chemistry, 27(1), 67–72.
[16] Heli, H., Zarghan, M., Jabbari, A., Parsaei, A., & Moosavi-Movahedi, A. A. (2010). Electrocatalytic oxidation of the antiviral drug acyclovir on a copper nanoparticles-modified carbon paste electrode. Journal of Solid State Electrochemistry: Current Research and Development in Science and Technology, 14(5), 787–795.
[17] Dorraji, P. S., & Jalali, F. (2016). Differential pulse voltammetric determination of nanomolar concentrations of antiviral drug acyclovir at polymer film modified glassy carbon electrode. Materials Science & Engineering. C, Materials for Biological Applications, 61, 858–864.
[18] Amouzadeh Tabrizi, M., & Shamsipur, M. (2015). A label-free electrochemical DNA biosensor based on covalent immobilization of salmonella DNA sequences on the nanoporous glassy carbon electrode. Biosensors & Bioelectronics, 69, 100–105.
[19] Zhao, Q.-L., Zhang, Z.-L., Bao, L., & Pang, D.-W. (2008). Surface structure-related electrochemical behaviors of glassy carbon electrodes. Electrochemistry Communications, 10(2), 181–185.
[20] Rahimnejad, M., Zokhtare, R., Moghadamnia, A. A., & Asghary, M.  (1397). Fabrication of electrochemical curcumin sensor based on carbon paste electrode. Applied Chemistry, 13(47), 91-104. in Persian
[21] Salavati, H., Taei, M., Rasouli, N., Zohoor, A., & Ashian, G. (1398). Application of Spinel-structured NiCuFe2O4 nanoparticles for determination of naproxen in the presence of acetaminophen. Applied Chemistry, 14(52), 119-134. in Persian
[22] Shetti, N. P., Malode, S. J., & Nandibewoor, S. T. (2012). Electrochemical behavior of an antiviral drug acyclovir at fullerene-C(60)-modified glassy carbon electrode. Bioelectrochemistry (Amsterdam, Netherlands), 88, 76–83.