شیمى کاربردى روز

شیمى کاربردى روز

تهیه و ارزیابی برون تن هیدروژل کایتوزان/آلژینات حاوی نانوذرات سلنیوم بارگذاری شده با دگزامتازون

نوع مقاله : مقاله علمی پژوهشی

نویسندگان
1 گروه نانوفناوری دارویی، دانشگاه علوم پزشکی زنجان، زنجان، ایران
2 مرکز تحقیقات نانو فناوری دارویی، دانشگاه علوم پزشکی زنجان، زنجان، ایران
چکیده
در مطالعه حاضر با بارگذاری نانوذرات سلنیوم حاوی دگزامتازون، در هیدروژل کیتوزان-آلژینات، یک داربست مهندسی بافت برای ضایعات مغزی به ویژه در بیماری سکته مغزی تهیه شده و خصوصیات فیزیکوشیمیایی آن بررسی گردید. آزمون های FTIR، DLS، XRD و AFM تهیه موفق میانگین قطر هیدرودینامیک و پتانسیل زتای 2/214 و 1/34+ میلی ولت بود. هیدروژل از پلیمرهای آلژینات و کیتوزان به دلیل برهم کنش یونی ساخته شد. آزمون های میکروسکوپ الکترونی روبشی و آنالیز عنصری نشان داد که نانوذرات سلنیوم در ساختار هیدروژل کیتوزان-آلژینات قرار گرفته اند. درصد احتباس و بارگذاری دارو به ترتیب6/3 ±1/93 و4/2±05/15درصد محاسبه شد. آزمون رهش دارو از هیدروژل در محیط بافر فسفات با pH 4/7 بررسی شد که نشان داد دگزامتازون از هیدروژل آزادسازی یکنواختی داشته و بعد از 160 ساعت حدود 70 درصد از دارو رهش یافته است. درصد تورم و درصد تخلخل هیدروژل به ترتیب 196% و 65% محاسبه شد. با در نظر گرفتن درصد همولیز زیر 5 درصد به دست آمده از آزمون همولیز برون تن، هیدروژل دارارای کاربرد بالقوه در زمینه مقاصد درمانی می باشد. با توجه به نتایج به دست آمده در مطالعه حاضر، می توان امیدوار بود که هیدروژل بارگذاری شده با نانوذرات سلنیوم حاوی دگزامتازون، داربست مناسبی برای مهندسی بافت در درمان سکته مغزی باشد.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Preparation and in vitro Evaluation of Chitosan / Alginate Hydrogel Containing Dexamethasone-Loaded Selenium Nanoparticles

نویسندگان English

Zahra Karami 1
Zahra Fazeli 1
Mehrdad Hamidi 2
1 Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
2 Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
چکیده English

In the present study, we developed a tissue engineering scaffold for brain injuries, particularly stroke treatment, by loading dexamethasone-containing selenium nanoparticles into a chitosan-alginate hydrogel. FTIR, DLS, XRD, and AFM analysis confirmed the successful preparation of dexamethasone-loaded selenium-chitosan nanocomposites (DEX@Cs-SeNPs). The nanocomposite's average hydrodynamic diameter and zeta potential were 214.2 nm and +34.1 mV, respectively. The hydrogel was then made from chitosan and alginate polymers due to the ionic interaction. Scanning electron microscopy and elemental mapping analysis confirmed the incorporation of selenium nanoparticles into the chitosan-alginate hydrogel. The encapsulation efficiency and drug loading capacity were 93.1±3.6% and 15.05±2.4%, respectively. Approximately 70% of the drug was released from hydrogel after 160 hours in phosphate-buffered saline. The percentage of swelling and porosity of the hydrogel was calculated as 196% and 65%, respectively. DEX@Cs-SeNPs@Cs/Alg Hydrogel showed a hemolysis percentage of 4.6% at a concentration of 2 mg/mL. Based on the results obtained in this study, DEX@Cs-SeNPs@Cs/Alg Hydrogel can be considered promising as a scaffold for brain tissue engineering.

کلیدواژه‌ها English

Hydrogel
Chitosan
Alginate
Selenium Nanoparticles
Dexamethasone
Stroke
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