شیمى کاربردى روز

شیمى کاربردى روز

تلفیق نانوکره های تو خالی طلا و آپتامر جهت سنجش دقیق و غیرتهاجمی کاربامازپین

نوع مقاله : مقاله علمی پژوهشی

نویسندگان
1 گروه آموزش شیمی، دانشگاه فرهنگیان، صندوق پستی 889-14665، تهران، ایران
2 دانشکده شیمی، دانشگاه سمنان، سمنان، ایران
چکیده
در این پژوهش، بر اساس تلفیق نانوکره‌های تو خالی طلا با آپتامر یک آپتاحسگر بسیار حساس و دقیق برای سنجش کاربامازپین پیشنهاد شده است. نانوکره‌های تو خالی طلا با مزایای منحصربفرد بر روی سطح الکترود کربن شیشه‌ای تثبیت شده و سپس توالی آپتامر با چگالی بالا بر روی سطح نانوکره تو خالی طلا متصل می‌شود. با افزودن کاربامازپین بر روی سطح سنجش تعبیه شده و تشکیل کمپلکس کاربامازپین/آپتامر، ممانعت فضایی سطح افزایش یافته و انتقال الکترون کاوشگر الکتروشیمیایی (فرو/فری سیانید) بر روی سطح محدود می‌شود. بدین ترتیب، کاربامازپین در محدوده 1 فمتومولار تا 900 نانومولار با حدتشخیص 3/333 آتومولار اندازه‌گیری می‌ گردد. عدم پاسخ در حضور سایر گونه‌ها با ساختارها و یا خواص مشابه و همچنین نتایج آنالیز رضایت‌بخش نمونه‌های مایعات زیستی انسانی گزینش‌پذیری بسیار بالای آپتاحسگر را نشان می‌دهد. استراتژی پیشنهادی جهت سنجش غیرتهاجمی کاربامازپین بر اساس دو شاخص بین المللی مطابق با اصول شیمی سبز تشخیص داده شده است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Combination of Hollow Gold Nanoparticles and Aptamer for Accurate and Non-invasive Measurement of Carbamazepine

نویسندگان English

Faeze Shahdoostfard 1
Maliheh Arhami 2
Alireza Asghari 2
Maryam Rajabi 2
1 Department of Chemistry Education, Farhangiyan University, P.O. Box 14665-889, Tehran, Iran
2 Department of Chemistry, Semnan University, Semnan, Iran
چکیده English

In this study, a highly sensitive and accurate aptasensor for carbamazepine detection is proposed based on the combination of hollow gold nanospheres with aptamers. Hollow gold nanospheres with unique advantages are immobilized on the surface of a glassy carbon electrode, and then the aptamer sequence is attached with high density on the surface of the hollow gold nanospheres. By adding carbamazepine to the embedded sensing surface and forming a carbamazepine/aptamer complex, the steric hindrance of the surface is increased and the electron transfer of the electrochemical probe (ferro/ferricyanid) on the surface is limited. Thus, carbamazepine is measured in the range of 1 femtomolar to 900 nanomolar with a detection limit of 3.3333 attomolar. The lack of response in the presence of other species with similar structures or properties, as well as the results of the satisfactory analysis of human biological fluid samples, indicates the very high selectivity of the aptasensor. The proposed strategy for the non-invasive measurement of carbamazepine was found to be in accordance with the principles of green chemistry in terms of two international indicators.

کلیدواژه‌ها English

Aptasensor
Carbamazepine
Hollow Gold Nanospheres
Non-invasive assay
Green chemistry
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