طراحی یک حسگر شیمیایی جدید برای شناسایی و اندازه‌گیری اسید سیتریک با استفاده از روش جا به جایی شناساگر

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

گروه شیمی، دانشگاه یاسوج، یاسوج، ایران

چکیده

در این تحقیق به معرفی یک حسگر رنگ‌سنجی برای اندازه‌گیری سیتریک اسید در نمونه حقیقی به روش سنجش جانشینی شناساگر پرداخته شد. این حسگر از واکنش تشکیل کمپلکس بین شناساگر کرومازورول S و یون گادولینیم ساخته شد. در pH بهینه 00/7، کرومازورول S (زرد رنگ )با گادولینیم (III) به نسبت 1 به 1 ترکیب شد و رنگ محلول کمپلکس کرومازورول S – گادولینیم به بنفش تغییرکرد. با افزایش سیتریک اسید به حسگر طراحی شده، سیتریک اسید جایگزین کرومازورول S شده و با یون گادولینیم به نسبت 1:1 واکنش می‌دهد. دلیل این جانشینی شناساگر با آنالیت در ترکیب با پذیرنده‌ی یونی گادولینیوم، ثابت تشکیل بزرگترکمپلکس سیتریک اسید –گادولینیم (86/6= logKF) نسبت به ثابت تشکیل کرومازورول S – گادولینیم (67/5= logKF) می‌باشد. دامنه خطی این اندازه‌گیری M 10-6×90/112 –10-6×27/2 می‌باشد و حد تشخیص روش (S/N=3)6- 10×08/2 مولار تعیین شد. جهت بررسی گزینش‌پذیری حسگر طراحی شده، اثر آنیون‌های دیگر از جمله پیروفسفات، سالیسیلیک اسید، پریدویک اسید، بنزوئیک اسید و ترکیبات دیگر،بر روی پاسخ حسگر در شرایط مشابه بررسی شد و تغییر رنگ قابل توجهی دیده نشد که نشاندهنده گزینش‌پذیری بالای حسگر برای اندازه‌گیری این آنالیت دارد. کارآیی روش با اندازه‌گیری سیتریک اسید در نمونه آب پرتقال با روش افزایش استاندادارمورد ارزیابی قرار گرفت. مدار منطقی مناسب برای این حسگر طراحی شده، XNOR می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Design of a chemical sensor for the detection and determination of citric acid using indicator displacement assay method

نویسندگان [English]

  • َAmin Hemmati
  • Habibollah Khajehsharifi
  • Zahra Arian
Department of Chemistry, Yasouj University, Yasouj, Iran
چکیده [English]

In this study, a colorimetric sensor was introduced to determine citric acid by the indicator displacement assay(IDA) method. The sensor was made from the complex formation reaction between the Chromeazurol S and the gadolinium ions in aqueous solutions. At optimum pH(pH =7.00), Chromeazurol S (yellow) was reacted with gadolinium (III) in a 1: 1 ratio, and the Chromeazurol S-gadolinium color complex solution changed to purple. With the addition of citric acid to the designed sensor, citric acid replaces Chromeazurol S and reacts with gadolinium ion in a 1: 1 ratio. The reason for the displacement of the indicator by the analyte in combination with the gadolinium ion acceptor is that it has a larger citric acid-gadolinium complex formation constant (logKF = 6.86) than the Chromeazurol S-gadolinium formation constant (logKF = 5.67). The linear range of this measurement and detection limit of this sensor (S/N=3) were determined 2.27×10-6 to 112.90×10-6 M and 2.08×10-6, respectively. To determine the selectivity of the designed sensor, the effect of different anions such as pyrophosphate, salicylic acid, periodic acid, benzoic acid, and other compounds on the sensor response under similar conditions was investigated, and no significant color change was observed. It was indicating that the sensor has a high selectivity to measure this analyte. The efficiency of the method was evaluated by measuring citric acid in the orange juice sample by the standard addition method.

کلیدواژه‌ها [English]

  • Chemosensor
  • Indicator Displacement Assay
  • Citric Acid
  • Chrome Azurol S
  • Gadolinium (III)
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