بررسی رفتار دارورسانی لیپوزوم آلیسین بر پایه فسفاتیدیل اتانول آمین و هیدروژل برپایه ثعلب

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 گروه مهندسی شیمی، دانشگاه علم و فناوری مازندران، بهشهر، ایران

2 گروه پتروشیمی و پلیمر، پژوهشگاه استاندارد، البرز، ایران

3 گروه مهندسی شیمی، دانشگاه آزاد اسلامی واحد آیت الله آملی، آمل، ایران

چکیده

در این تحقیق پوشش لیپوزوم بر پایه فسفاتیدیل اتانول آمین ( با روش بنگهام) و هیدروژل بر پایه ثعلب، با هدف بررسی ماندگاری داروی آلیسین تولید و بارگذاری شدند. ساختار لیپوزوم وهیدروژل با FT-IR و SEM بررسی شدند. با در نظر گرفتن متغیرهای نسبت فسفاتیدیل اتانول آمین به کلسترول، نسبت حلال فاز آلی به حلال فاز آبی و زمان تشکیل لیپوزوم، یک مدل جهت پیش‌بینی بازده کپسوله‌سازی آلیسین در لیپوزوم ارائه شد. شرایط بهینه میزان فسفاتیدیل اتانول آمین به کلسترول 8:1، نسبت فاز آلی- فازآبی 1 :8/4 و زمان تشکیل min158 با استفاده از روش سطح پاسخ و نرم‌افزار دیزاین اکسپرت 12 پیش‌بینی شد که منجر به بازده 99/98 % می‌شود. برای پوشش هیدروژل با پایه ثعلب مقدار بیشینه کپسوله‌سازی 98/74% در زمان تشکیل min140، میزان آکریلیک اسید ml3 و میزان N،N’ - متیلن‌بیس‌آکریل‌آمید g01/0 بدست آمد. روند رهایش آلیسین از پوشش لیپوزوم و هیدروژل نشان داد که هردو پوشش می‌توانند کاربری مناسبی برای انتقال دارو در شرایط محیطی با pH حدود 7.4 داشته باشند. بررسی سینتیک انتشار آلیسین از لیپوزوم و هیدروژل براساس مدل‌های واکنش درجه اول، مدل هیگوچی و معادله هیکسون-کراول و مدل ویبول نشان داد که رفتار رهایش آلیسین از این دو پوشش به مدل ویبول نزدیکتر است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of drug delivery of allicin liposome based on phosphatidylethanolamine and salep-based hydrogel

نویسندگان [English]

  • Romisa Mohammad Gholinezhad 1
  • Nooshin Gholipour Zanjani 2
  • Arash Kamran Pirzaman 1
  • Behzad Zahed 3
1 Department of Chemical Engineering, University of Science and Technology of Mazandaran, Behshahr, Iran
2 Chemistry and Petrochemistry Research Center, Standard Research Institute, Iran
3 Department of Chemical Engineering, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran
چکیده [English]

In this study, liposome coatings based on phosphatidylethanolamine (by Bingham method) and hydrogel based on salep were produced and loaded with the aim of allicin shelf life study. The structure and morphology of Liposome and hydrogel were studied by FT-IR and SEM. Considering design variables of phosphatidylethanolamine to cholesterol ratio, organic phase solvent to aqueous solvent ratio and liposome formation time, a model was proposed to predict the allicin encapsulation efficiency in liposomes. The optimum condition was achieved by Response Surface Method and software of Design-Expert v.12 where the amount of phosphatidylethanolamine-to-cholesterol 8:1, organic phase to water phase ratio of 4.8:1 and formation time of 158 minutes lead to a yield of 98.99%. For salep-based hydrogel coatings, the maximum encapsulation value was 74.98% at 140 min formation time, while the amount of acrylic acid was 3 ml and the amount of N, N '- methylenebisacrylamide was 0.01 gr. The release behavior of allicin from the liposome coating and the hydrogel coating showed both of them are suitable for drug delivery in the media with pH of 7.4. Investigation of allicin release kinetics from liposomes and hydrogels based on first-order reaction models, Higuchi model and Hixson-crowell equation and Weibull model indicated that Weibull can better fit the experimental data.

کلیدواژه‌ها [English]

  • Allicin
  • Liposome
  • Hydrogel
  • Salep
  • Encapsulation
  • Released Control
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