بررسی نتایج حاصل از مشتقات دارویی کرومن و تاثیر آنها بر سرطان کولون

نوع مقاله : مقاله علمی پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه حکیم سبزواری، سبزوار، ایران

2 گروه شیمی آلی، دانشکده شیمی، دانشگاه مازندران، بابلسر، ایران

چکیده

سرطان یک عارضه بزرگ در جامعه انسانی است و در کشورهای کمتر و یا حتی بیشتر توسعه یافته از نظر اقتصادی نیز دیده می‌شود. سرطان کولون در اثر رشد سلول‌های سرطانی در روده بزرگ به وجود می‌آید که دلیل اصلی آن رشد غیرقابل کنترل سلول‌ها در ناحیه مورد نظر است. در این تحقیق دو گروه از ترکیبات پایدار کرومنی مورد مطالعه با نرم افزارهای بیوانفورماتیکی، انتخاب گردید و اثر سمیت آنها و نوع مرگ سلولی توسط آنها بر رده سلولی سرطانی HT-29 که مربوط به سرطان کولون می‌باشد، بررسی شد. همچنین، سمیت2-آمینو-4(4-کلرو فنیل)-5-اکسو-H4, H5-پیرانو] 2،3-C [ کرومن 3-کربونیتریل (S1) و 2-آمینو-4(4-برومو فنیل)-5-اکسو-H4, H5-پیرانو] 2،3-C [ کرومن 3-کربونیتریل (S2)که پایداری مناسبی داشتند تعیین و در غلظت‌های 1 ،2 ،3 ،5 ،10و20 میکروگرم در میلی‌لیتر و یک گروه کنترل بر روی رده های سلولی سرطانی HT-29 در مقایسه با داروی شیمی‌درمانی کمپتوسار با غلظت‌های مشابه داروی شیمی‌درمانی به کمک تست MTT مورد بررسی قرار گرفته اند. آنالیز داده ها با استفاده از نرم افزار آماری8 Graphpad Prism و آزمون ANOVA- دوطرفه انجام شد و در نهایت نوع مرگ سلولی ایجاد شده توسط تکنیک فلوسایتومتری مورد بررسی قرار گرفت. نتایج بدست آمده نشان داد که مشتقات کرومنی در غلظت 20 میکروگرم بر میلی لیتر با ایجاد آپوپتوز در سلول‌های سرطانی HT-29 می‌توانند به عنوان کاندیدای مناسبی جهت اثر بر سرطان کولون مورد بررسی‌های بیشتر قرار گیرند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of the results of chromen drug derivatives and their effect on colon cancer

نویسندگان [English]

  • reihaneh sabbaghzadeh 1
  • morvarid moradi 1
  • majid momeni-moghadam 1
  • behrooz maleki 2
1 Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
2 Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
چکیده [English]

Cancer is a huge phenomenon in human society that it observe in developing countries and even more developed countries in terms of economic as well. Colon cancer is created by increasing cancer cells that its main reason is the uncontrollable growth of cells in Colon. In this research, two groups of stable chromene compounds which was calculated in the previous study using bioinformatics software selected and their toxicity effect and the kind of cell death that they cause investigated on HT-29 cancer cell line, which is related to colon cancer. Also, toxicity of 2-amino-4-(4-chlorophenyl)-5-oxo-4H,5H-pyrano [2,3-c] chromene -2-carbonitrile (S1), 2-amino-4-(4-bromophenyl)-5-oxo-4H,5H-pyrano [2,3-c] chromene -2-carbonitrile (S2) which had an appropriate stable determined. At concentrations of 1, 2, 3, 5, 10 and 20 μg / ml, a control group was performed on HT-29 cancer cell lines in comparison with the chemotherapeutic drug Campusar with similar concentrations of the chemotherapy drug using MTT assay. Analysis of data was done by statistical software Graphpad Prism 8 and ANOVA test and ultimately, the type of cell death which created by Flow cytometry technique was examined. The results showed that chromium derivatives by causing apoptosis in HT-29 cancer cells could be further studied as a suitable candidate for the effect on colon cancer.

کلیدواژه‌ها [English]

  • Annexin-V
  • HT-29
  • molecular modeling
  • Colon Cancer
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